2015 Fiscal Year Final Research Report
Engineering of photo-activated ion channels
| Project/Area Number |
25840055
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | The Graduate School for the Creation of New Photonics Industries |
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Principal Investigator |
Hirano Minako 光産業創成大学院大学, 光産業創成研究科, 講師 (80585167)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | イオンチャネル / 光感受性 |
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| Outline of Final Research Achievements |
We aimed to engineer a photo-sensitive K+ channel and Ca2+ channel by modifying a KcsA channel to regulate cell functions by light. 1) Chimera mutants which had photo-sensitive LOV domains at a cytoplasmic domain of the KcsA showed various activities depending on length of linkers, and the mutant which had a linker consisting of 35 amino acids had higher activity in dark than under blue light. 2) Electrostatic state of E146 at the cytoplasmic domain in the KcsA affected activities. 3) Some mutations in a selectivity filter site in KcsA changed it to be Ca2+ permeable.
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| Free Research Field |
生物物理学
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