2014 Fiscal Year Final Research Report
Studying diversity of cichlid fish community using environmental DNA analysis
| Project/Area Number |
25840144
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biodiversity/Systematics
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| Research Institution | Ryukoku University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 環境DNA / シクリッド魚類 / マラウイ湖 / ITS / D-loop / 定量PCR |
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| Outline of Final Research Achievements |
The environmental DNA technique was applied to cichlid fish community in Lake Malawi to establish a method for measuring divesity of the community. Sequence data of D-loop and ITS regions were collected from 103 sympatric fish species at the beggining of the study period. Using the sequence data, an eDNA primer targetting D-loop region of cichlids was designed, with which the efficient sampling methods specific to the focal lake was established. Water samples were collected from 12 sites of 6 district of the lake, which differ from each other in cichlid diversity. As interspecific variation in DNA sequence was found to be so poor, I am now trying to design another universal primers that amplify ITS region, with which cichlid diversity will be estimated.
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| Free Research Field |
魚類生態学
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