Functional analysis of ROS signal regulator "RAM1" in plant defense

2014 Fiscal Year Final Research Report

• Project/Area Number: 25850034
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant protection science
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: MATSUI Hidenori 独立行政法人理化学研究所, 環境資源科学研究センター, 特別研究員 (20598833)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: MAMP / リン酸化タンパク質 / 細胞死 / 抵抗性 / R遺伝子

Outline of Final Research Achievements

To identify novel plant immune components, we performed advanced phosphoproteomics approaches and identifed the mutant of “MAMP-responsive phosphoprotein for appropriated ROS kinatics 1”. MARK1 plays an important role for proper regulation of MAMP responses, but also functions as a regulator of cell death during R-gene mediated resistance in Arabidopsis thaliana. To reveal the molecular function of MARK1 protein, we performed yeast two hybrid assay to identify MARK1 interacting proteins (MIPs). We successfully isolated several MIPs, including mip1 which shows enhanced cell death phenotype, similar to mark1. These data suggest that MARK1-MIP1 interaction may regulate cell death during R-gene mediated resistance and this interaction likely play a role in plant immunity.

Free Research Field

植物病理学