Mechanisms underlying target specificity changes of DNA methyltransferase, and its application to epigenome breeding

2015 Fiscal Year Final Research Report

• Project/Area Number: 25850049
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: The University of Tokyo
• Principal Investigator: Yano Hirokazu 東京大学, 新領域創成科学研究科, 特任助教 (20646773)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: エピゲノム / トランスクリプトーム / DNAメチル化 / ピロリ菌 / メチローム / 制限修飾系

Outline of Final Research Achievements

We obtained basic knowledge that contributes to ‘epigenome breeding’ wherein we manipulate bacterial phenotypes using transcriptome-modulation capability of DNA methyltransferases. We demonstrated that a Type II DNA methyltranferase in a H.pylori strain contributes to adaptive phenotypes through gene regulation. Furthermore we found that it is possible to modulate transcriptome of E. coli by introducing sequence-specific DNA methyltransferase genes to E. coli.

Free Research Field

応用微生物学