2014 Fiscal Year Final Research Report
The development of plant alkaloid production system by using bacterial platform.
| Project/Area Number |
25850054
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Ishikawa Prefectural University |
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Principal Investigator |
MINAMI HIROMICHI 石川県立大学, 生物資源環境学部, 准教授 (90433200)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Keywords | 植物アルカロイド / 発酵 / バイオテクノロジー / 応用微生物 / 酵素反応 |
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| Outline of Final Research Achievements |
We examined the side-chain modification of reticuline by introducing glycosyltransferases or sulfotransferases into reticuline-producing system.
In the reticuline-producing strain containing sulfotransferase (SULT1E1) from human, reticuline 3’-O-sulfate was produced from glucose at a yield of 222 mg/L. Furthermore, we showed that UDP-glucuronosyltransferases (UGT2B1) from rat and mouse catalyze the addition of a glucuronic acid moiety to reticuline.
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| Free Research Field |
農学
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