Regulation of inflammation responses by HDL formation

2014 Fiscal Year Final Research Report

• Project/Area Number: 25850070
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry
• Research Institution: Kyoto University (2014); The University of Tokushima (2013)
• Principal Investigator: NAGAO Kohjiro 京都大学, 工学(系)研究科(研究院), 助教 (40587325)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: コレステロール

Outline of Final Research Achievements

ABCA1 mediates the efflux of cholesterol and phospholipids into apoA-I to form HDL, which is important in not only the prevention of atherosclerosis but also the regulation of inflammation responses. To develop a novel method for the evaluation of HDL formation, we prepared an apoA-I-POLARIC by labeling the apoA-I variant with a hydrophobicity-sensitive fluorescence probe that detects the environmental change around apoA-I during HDL formation. THP-1 macrophage-mediated HDL formation could be measured using apoA-I-POLARIC. These results demonstrate that HDL formation by ABCA1-expressing cells can be specifically detected by sensing hydrophobicity change in apoA-I, thus providing a novel method for assessing HDL formation.

Free Research Field

細胞生物学