2014 Fiscal Year Final Research Report
Regulation of inflammation responses by HDL formation
Project/Area Number |
25850070
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Applied biochemistry
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Research Institution | Kyoto University (2014) The University of Tokushima (2013) |
Principal Investigator |
NAGAO Kohjiro 京都大学, 工学(系)研究科(研究院), 助教 (40587325)
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Keywords | コレステロール |
Outline of Final Research Achievements |
ABCA1 mediates the efflux of cholesterol and phospholipids into apoA-I to form HDL, which is important in not only the prevention of atherosclerosis but also the regulation of inflammation responses. To develop a novel method for the evaluation of HDL formation, we prepared an apoA-I-POLARIC by labeling the apoA-I variant with a hydrophobicity-sensitive fluorescence probe that detects the environmental change around apoA-I during HDL formation. THP-1 macrophage-mediated HDL formation could be measured using apoA-I-POLARIC. These results demonstrate that HDL formation by ABCA1-expressing cells can be specifically detected by sensing hydrophobicity change in apoA-I, thus providing a novel method for assessing HDL formation.
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Free Research Field |
細胞生物学
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