Reaction mechanism of hydrazine synthesis system of anaerobic ammonium oxidation

2014 Fiscal Year Final Research Report

• Project/Area Number: 25850076
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry
• Research Institution: Sojo University
• Principal Investigator: HIRA Daisuke 崇城大学, 生物生命学部, 准教授 (00569890)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: anammox / ヘムタンパク質 / ヒドラジン合成

Outline of Final Research Achievements

In this research, we attempted to elucidate the reaction mechanism of hydrazine synthesis system consisting of hydrazine synthase (HZS) and heterodimer cytochrome c (NaxLS) of anammox bacteria by the spectroscopic measurement and X-ray crystal structure analysis.
First, by site-specific mutagenesis to NaxLS, it was revealed that the reduction of NaxLS is essential for hydrazine synthesis and strongly suggested that the electron transfer from NaxLS to HZS is essential during the course of the reaction. Second, it was revealed that HZS has β-propeller like structure by the X-ray crystal structure analysis of HZS. Finally we revealed the crystallization conditions of HZS and NaxLS complex. We are now attempting the crystallographic analyses of the complex.

Free Research Field

金属蛋白質科学