2015 Fiscal Year Final Research Report
Construction of basis of molecular breeding for male sterile trees in Cryptomeria japonica and development of selection markers
| Project/Area Number |
25850116
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Forest science
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| Research Institution | Niigata University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 連鎖解析 |
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| Outline of Final Research Achievements |
Using information from this high-density map of Cryptomeria japonica, we newly located three male-sterile genes (ms2, ms3 and ms4) on the 5th, 1st and 4th linkage groups, respectively, and constructed partial linkage maps around these loci. In addition, we have constructed partial linkage maps surrounding a male sterility gene (ms1) in four families (TO13S, S3T67, F1N4 and T5). In all four families, the accuracy with which male-sterile trees could be identified using the closest markers was more than 96.0 %. We also developed an allele-specific PCR marker for identifying male-sterile trees in the TO13S family from which male-sterile seedlings are produced.
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| Free Research Field |
林木遺伝育種学
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