2015 Fiscal Year Final Research Report
Monitoring of pesticide residues in farmed fish by bioluminescent reporter gene assay
| Project/Area Number |
25850135
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Aquatic bioproduction science
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| Research Institution | Tokyo University of Marine Science and Technology |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | ルシフェラーゼ / Two-hybrid system / ティラピア / EPC細胞 / AhR / PXR / CYP1B1 / ヘテロ二量体 |
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| Outline of Final Research Achievements |
Pesticides have been prohibited to use for aquaculture, but still detected from farmed fish. Although pesticide residues in fish are monitored by GC/MS or LC/MS, these are not used routinely in most fish farming due to their high cost operation and time requirement for sample preparation.
CYPs and P-gp play important roles in drug metabolism. The transcriptions of CYPs and P-gp are regulated by drug receptors such as AhR and PXR. Here, we investigated whether the cell that contains these molecules in control of a reporter gene is applicable to monitoring of pesticide residues in fish.
The heterodimerization between AhR and Arnt was investigated by two-hybrid system on fish cells. AhR forms a heterodimer with Arnt in the presence of pesticides. Moreover, the expression of CYP1B1 in hepatocytes was induced by pesticides. These results suggest that AhR/Arnt heterodimerization becomes reliable cellular reporter for monitoring the pesticide residues in fish.
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| Free Research Field |
水族病態生理学
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