Development of a novel vaccine to control virulent strains of Marek's disease virus: enhancement of vaccine efficacy by modulating the host immunosuppression pathway

2014 Fiscal Year Final Research Report

• Project/Area Number: 25850194
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Hokkaido University
• Principal Investigator: MURATA Shiro 北海道大学, (連合)獣医学研究科, 助教 (10579163)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: マレック病 / マレック病ウイルス / 組換えワクチン / PD1 / PD-L1 / 免疫抑制因子

Outline of Final Research Achievements

Field isolates of Marek’s disease virus (MDV) have tended to increase in virulence, and therefore, the development of more effective vaccines would be desirable. To develop a novel vaccine, we focused on the PD1/PD-L1 pathway that contributes to the immune suppression, and reconstituted the recombinant MDVs that carry the soluble-PD1 gene to competitively inhibit the immunosuppression regulated by the endogenous PD1. The recombinant vaccine expressing soluble PD1 induced higher production of interferon γ in the immune cells from the vaccinated chickens, indicating that this vaccine potentially possesses an ability to induce more effective cell-mediated immunity in chickens.

Free Research Field

獣医伝染病学