Regulation of cell-surface MHC-II in dendritic cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 25860049
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: Okayama University
• Principal Investigator: FURUTA Kazuyuki 岡山大学, 医歯(薬)学総合研究科, 准教授 (50644936)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 樹状細胞 / MHC class II / Ubiquitin / Endocytosis / 小胞輸送

Outline of Final Research Achievements

Major histocompatibility complex class II (MHC-II) which expresses on dendritic cells presents antigenic peptides to CD4 T cells. The MHC-II-mediated antigen presentation initiates acquired immunity. Although the cell-surface expression of MHC-II is tightly regulated, the regulation mechanism remains to be clarified. In this study we obtained the following results: (1) Internalizing MHC-II from cell-surface is found to be targeted to the early endosome where E3 ligase MARCH-I is localized. As a result, MHC-II is ubiquitinated and targeted for lysosomal degradation. (2) Recycling of internalized MHC-II to cell-surface is found to be regulated by Rab small G protein family, Rab11 and Rab35.

Free Research Field

医歯薬学