2014 Fiscal Year Final Research Report
Sphingomyelin Synthase 2, but not Sphingomyelin Synthase 1, is Involved in HIV-1 Envelope-mediated Membrane Fusion
| Project/Area Number |
25860055
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | Teikyo University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 脂質 / ヒト免疫不全ウィルス |
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| Outline of Final Research Achievements |
In the present study, we examined the roles of SM synthase (SMS) in HIV-1 Env-mediated membrane fusion using a cell-cell fusion assay with HIV-1 mimetics and their target cells. We employed reconstituted cells as target cells that stably express SMS1 or SMS2 in SMS-deficient cells. Fusion susceptibility was approximately five-fold higher in SMS2-expressing cells (not in SMS1-expressing cells) than in SMS-deficient cells. The enhancement of fusion susceptibility observed in SMS2-expressing cells was reversed and reduced by SMS2 knockdown. We also found that catalytically non-active SMS2 promoted membrane fusion susceptibility. Moreover, SMS2 co-localized and was constitutively associated with the HIV receptor/co-receptor complex in the plasma membrane. Taken together, our research provides insight into a novel function of SMS2: regulation of HIV-1 Env-mediated membrane fusion via actin rearrangement.
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| Free Research Field |
脂質
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