Analyses of mechanisms of regulation of cell fusion and formation of tumor-associated multinucleated giant cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 25860293
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: Nagoya University
• Principal Investigator: Mii Shinji 名古屋大学, 医学(系)研究科(研究院), 助教 (80646505)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: CD109 / 破骨細胞 / 間葉系間質細胞 / 骨肉腫

Outline of Final Research Achievements

In this study, immunohistochemical analysis revealed CD109 expression in human and murine osteoclasts in bones and osteoclast-like multinucleated giant cells in human osteosarcoma and giant cell tumor.
In addition, we performed co-culture experiments using primary osteoblasts and pre-osteoclasts isolated from either wild-type or CD109-deficient mice. In this in vitro co-culture experiments, CD109-deficient pre-osteoclasts failed to multinucleate by cell fusion.
These findings suggest that CD109 plays a role in cell fusion of osteoclasts and in formation of osteoclast-like multinucleated giant cells.

Free Research Field

実験病理学