2014 Fiscal Year Final Research Report
Role of long non-coding RNA in regulation of inflammatory cytokine expression
| Project/Area Number |
25860307
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Experimental pathology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
CHIBA Tomoki 東京医科歯科大学, 医歯(薬)学総合研究科, 特任研究員 (00645830)
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| Co-Investigator(Renkei-kenkyūsha) |
ASAHARA Hiroshi 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (70294460)
KATO Tomomi 東京医科歯科大学, 大学院医歯学総合研究科, 技術補佐員
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 非コードRNA / 炎症性サイトカイン |
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| Outline of Final Research Achievements |
Recent advantages of transcriptome analyses revealed that non-coding RNAs, especially long non-coding (lnc) RNAs, are transcribed across genome and involved in various aspects of biological processes. I identified novel lncRNA that is essential for inflammatory cytokine expression. IL-6 and GM-CSF expression was severely reduced by lncRNA silencing upon LPS stimulation. LncRNA was mainly localized in cytoplasm and did not influence NF-kappaB activation. Further, to understand physiological roles, lncRNA transgenic and knockout mice were successfully generated by transposon and CRISPR/Cas9 system, respectively.
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| Free Research Field |
免疫学
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