Identification and functional analyses of the target genes of transcription factor Spi-B in Peyer's patch M cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 25860353
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Immunology
• Research Institution: The University of Tokyo
• Principal Investigator: SATO SHINTARO 東京大学, 医科学研究所, 助教 (80447333)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 粘膜免疫 / M細胞

Outline of Final Research Achievements

To reveal the functional mechanism of M cells, we tried to identify the target gene cluster of transcription factor Spi-B, which was essential to M-cell differentiation. Finally, we found three genes as an M-cell specific expression gene. Among them, we could analyze one candidate molecule (Cand1) about antigen uptake ability in vivo using the Cand1-deficient mice. As a result, the uptake of beads and Yersinia enterocolitica significantly decreased in Cand1-deficient mice when compared with a wild type mice. These results suggest that Cand1 is associated with the expression and/or activation of beta 1 integrin, which is believed as the receptor for Yersinia.

Free Research Field

粘膜免疫学