2015 Fiscal Year Final Research Report
Aberrant hydroxymethylation in human liver cancer
| Project/Area Number |
25860521
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NAGAE Genta 東京大学, 先端科学技術研究センター, 特任講師 (10587348)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 肝臓病学 / エピジェネティクス |
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| Outline of Final Research Achievements |
Methylation profiles of hepatocellular carcinoma (HCC) have revealed aberrant hypermethylation and hypomethylation, which form four subgroups. However, molecular significance of aberrant hypomethylation in HCC remains poorly understood. Here, we performed comprehensive profiling of cytosine methylation and hydroxymethylation (5-hmC) for the TET1-upregulated HCCs and clarified the aberrant deposition of 5-hmC in a genome-wide manner. Knockdown of endogenous TET1 in hepatoma cell lines decreased hmC deposition with cell growth suppression. Collectively, these results indicate the novel function of methylcytosine dioxygenase TET1 that promotes cell proliferation though epigenetic dysregulation of the oncogenic targets during hepatocarcinogenesis.
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| Free Research Field |
肝臓病学
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