Analysis of drug sensitivity and resistance by single cell-based genetic assay and intratumoral profiling of gastric cancer

2015 Fiscal Year Final Research Report

• Project/Area Number: 25860542
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Kyushu University
• Principal Investigator: Arita Shuji 九州大学, 医学(系)研究科(研究院), 助教 (20544935)
• Co-Investigator(Renkei-kenkyūsha): ARIYAMA Hiroshi 九州大学病院, 血液・腫瘍内科, 助教 (80713437)
• Research Collaborator: NAKANO Michitaka ; TANAKA Mamoru
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 大腸がん / 単細胞解析 / がん幹細胞 / TGF-β

Outline of Final Research Achievements

This research program was based on a concept to analyze intratumor heterogeneity of gastrointestinal cancers by single cell-based genetic assay. Although originally planned to analyze intratumor clonal transision and drug resistance of gastric cancer, it was difficult to establish an assay of human gastric cancer. We consequently conducted experiments on human colorectal cancer to analyze cancer stemness and intratumor clonality. We achieved an experimental system of organoid formation from patient-derived CD44-positive colorectal cancer cells. Furthermore, CD44-negative cells also formed organoids under a condition of epidermal-mesenchimal transision. TWIST1 and TGF-beta signal pathway were involved in this phenomenon.

Free Research Field

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