• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2015 Fiscal Year Final Research Report

Analysis of the pathogenesis in hypertophic cardiomyopathy by using zebrafish models.

Research Project

  • PDF
Project/Area Number 25860589
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Cardiovascular medicine
Research InstitutionKanazawa University

Principal Investigator

Hodatsu Akihiko  金沢大学, 医学系, 協力研究員 (00623662)

Co-Investigator(Renkei-kenkyūsha) SEGUCHI OSAMU  国立循環器病研究センター, 病院, 医師 (60570869)
Project Period (FY) 2013-04-01 – 2016-03-31
Keywords肥大型心筋症 / ゼブラフィッシュ / 遺伝子変異
Outline of Final Research Achievements

MYBPC3 antisense morpholino oligonucleotide (MO) and MYBPC3 mRNA were injected in one cell stage zebrafish embryos to assess the functional consequences of the MYBPC3 mutations found in human hypertrophic cardiomyopathy patients. Wild type MYBPC3 mRNA, wild type and A745D mutant mRNA, or double mutant mRNA (A745D and R815Q mutants) were co-injected. They corresponds wild type, single mutation (heterozygote) and compound heterozygote, respectively. Ventricular dimension
and heart rate were similar between the MYBPC3 MO group and the MYBPC3 MO with WT mRNA group or the MYBPC3 MO with Ala745Asp mRNA and WT mRNA group, whereas ventricular dimension and heart rate were significantly increased in the MYBPC3 MO with Ala745Asp mRNA and Arg815Gln mRNA group compared with the MYBPC3 MO group.

Free Research Field

循環器内科

URL: 

Published: 2017-05-10  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi