Clarification of the mechanism how human cardiac progenitor cells differentiate into cardiomyocytes.

2014 Fiscal Year Final Research Report

• Project/Area Number: 25860603
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular medicine
• Research Institution: Okayama University
• Principal Investigator: YOSHIDA Masashi 岡山大学, 医歯(薬)学総合研究科, 助教 (70532761)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 心臓内幹細胞 / 内皮細胞 / DNAマイクロアレイ

Outline of Final Research Achievements

We compared expressed genes in human cardiac progenitor cells before and after cardiac differentiation by DNA microarray. We differentiated human cardiac progenitor cells into cardiomyocytes by co-culture those cells with neonatal rat ventricular myocytes. This comparison revealed that a set of genes which promote cells into endothelial cells was expressed in human cardiac progenitor cells. But a set of genes which are known to promote embryonic stem cells into cardiomyocytes was not able to be compared because DNA microarray chips reacted with rat genes. Careful interpretation may be need to understand the mechanism how human cardiac progenitor cells differentiate into cardiomyocytes with these results. On the other hand, we found that human progenitor cells can be transdifferentiate into cardiomyocytes by co-culture those cells with human endothelial cells

Free Research Field

再生医療　心不全