2014 Fiscal Year Final Research Report
Development of the kidney regeneration therapy using adipose tissue-derived stromal cells .
| Project/Area Number |
25860672
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Kidney internal medicine
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 脂肪由来幹細胞 / 再生 / 低血清培養 |
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| Outline of Final Research Achievements |
BACKGROUND AIMS:Administration of rat adipose tissue-derived stromal cells (ASCs)potentially can improve several tissue injuries. The present study investigatedwhether rat ASCs could improve peritoneal inflammation in Zy/scraping peritonitis. METHODS:Rat ASCs were injected intraperitoneally on a daily basis in rats with Zy/scraping peritonitis.RESULTS:Peritoneal inflammation accompanied by accumulation of inflammatory cells and complement deposition was suppressed by day 5 afterinjection of rat ASCs. The peritoneal mesothelial layer in Zy/scrapingperitonitis with rat ASC treatment was restored compared withtheperitoneal mesothelial layer without rat ASC treatment. In vitro assaysshowedincreased cellular proliferation of rat mesothelial cells combined with ratASCs by co-culture assays. Hepatocyte growth factor was released from rat ASCs, andadministration of recombinant hepatocyte growth factor increased rat mesothelial cell proliferation.
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| Free Research Field |
再生医療
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