Tissue remodeling by microglia for a novel therapeutic strategy in chronic cerebral ischemia

2014 Fiscal Year Final Research Report

• Project/Area Number: 25860702
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurology
• Research Institution: Niigata University
• Principal Investigator: KANAZAWA Masato 新潟大学, 医歯学総合病院, 助教 (80645101)
• Co-Investigator(Renkei-kenkyūsha): SHIMOHATA Takayoshi 新潟大学, 脳研究所, 准教授 (60361911) ; TAKAHASHI Tetsuya 新潟大学, 医歯学総合病院, 助教 (20515663)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 脳虚血 / 細胞療法 / ミクログリア / 組織リモデリング / 血管リモデリング

Outline of Final Research Achievements

We evaluated the cellular proliferation in rats subjected to focal cerebral ischemia. We observed proliferated endothelial cells and pericytes, and increased numbers of microglia even though in the ischemic core, and angiogensis in the peripheral zone of the ischemic core at 7 and 14 days after cerebral ischemia. In vitro primary cells revealed that vascular endothelial growth factor was secreted from the microglia after oxygen-glucose deprivation (OGD). Protective growth factor progranulin was also secreted from the neuronal cells and microglia after OGD. Finally, we demonstrated the therapeutic potential of microglia against chronic focal cerebral ischemia. Seven days after ischemia, intraarterial injection of microglia stimulated OGD via carotid artery improved motor outcome compared to injections of astrocytes and phosphate buffer solution. Microglial transplantation may be a novel therapeutic strategy that provides tissue remodeling after chronic cerebral ischemia.

Free Research Field

脳虚血