Development and verification of implantable pancreatic epithelial cells from human pluripotent stem cell

2014 Fiscal Year Final Research Report

• Project/Area Number: 25860747
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Metabolomics
• Research Institution: Kyoto University
• Principal Investigator: TOYODA Taro 京都大学, iPS細胞研究所, 特定拠点助教 (60593530)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: iPS細胞 / ES細胞 / PDX1 / NKX6.1 / 膵臓 / 糖尿病

Outline of Final Research Achievements

Pancreatic bud/epithelial cells (NKX6.1+ cells) are considered as pancreas committed cells. Induction of the NKX6.1+ cell is a critical step for the generation of pancreatic cells from human pluripotent stem cells. However the induction protocol is not established. In this study we demonstrated that cellular aggregation is a key factor for induction of NKX6.1+ cells. Our generated NKX6.1+ cells expressed marker genes for pancreatic bud cells and could differentiate into pancreatic epithelia-like structures and beta cellｓ which secrete insulin in response to glucose level after implantation. Our new protocol is useful for basic research and clinical application using pancreatic cells.

Free Research Field

再生医学