An early and non-invasive diagnosis of histiocytic necrotizing lymphadenitis

2014 Fiscal Year Final Research Report

• Project/Area Number: 25860868
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pediatrics
• Research Institution: Kyushu University
• Principal Investigator: MASATAKA Ishimura 九州大学, 医学(系)研究科(研究院), 助教 (10448417)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 組織球性壊死性リンパ節炎 / 菊池病 / 早期診断 / インターフェロン / インターフェロン誘導遺伝子

Outline of Final Research Achievements

Histiocytic necrotizing lymphadenitis (HNL), also called Kikuchi-Fujimoto disease, is a benign, self-limiting inflammatory disease with fever and painful cervical lymphadenopathy of unknown etiology. A lymph node biopsy is required for the definitive diagnosis because of no specific symptoms or laboratory findings for HNL. We investigated genes specifically expressed in the patients by analyzing whole transcriptome using microarray analysis of peripheral blood mononuclear cells (PBMC). The expression levels of the up-regulated genes by microarray were verified by quantitative PCR. H The discriminant analysis using the expression levels of these five genes distinguished HNL with 84 % accuracy. An analysis of the gene expression profile of PBMC may provide a rapid non-invasive diagnosis.

Free Research Field

血液・免疫