Identification of gene mutation profiles related to responses to radiation therapy

2015 Fiscal Year Final Research Report

• Project/Area Number: 25861066
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: Gunma University
• Principal Investigator: Oike Takahiro 群馬大学, 医学部附属病院, 助教 (10643471)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 遺伝子変異 / 放射線感受性

Outline of Final Research Achievements

The present study aimed to identify gene mutation profiles in human cancer that correlate with sensitivity to radiation therapy. From a prospective and retrospective cohort of patients with uterine cervical cancer treated by radiation therapy, we obtained tumor biopsy samples before initiation of radiation therapy. We extracted DNA from the formalin-fixed and paraffin-embedded tumor biopsy samples. Using a next-generation sequencer, we performed target-capture sequencing of the extracted DNA and analyzed mutational status in 50 known cancer-related genes. Consequently, we identified several genes including FGFR family genes and SMAD4 whose mutational status can affect sensitivity to radiation therapy.

Free Research Field

放射線腫瘍学