2015 Fiscal Year Final Research Report
Analysis of induced cancer stem-like sphere cells from de-differentiated human hepatocellular carcinoma cell lines to examine the molecular targets for the cancer therapy
| Project/Area Number |
25861192
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 癌 |
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| Outline of Final Research Achievements |
Cancer stem cells (CSCs) are thought to play important roles in therapy-resistance. In this study, we induced cancer stem-like cells from hepatocellular carcinoma (HCC) cell lines using a unique medium, and examined their potential for resistance to anti-cancer drugs. Poorly differentiated HCC derived cell lines efficiently formed spheres of cells, but well-differentiated HCC-derived cells did not. Sphere cells showed increased stemness marker levels. We observed more CD44 variant-positive cells in Sphere cells than in parental cells. The cell viability of Sphere cells was significantly higher than that of parental cells in the presence of several anti-cancer drugs. Sphere cells showed higher ABCG2 and HIF1A mRNA expression and lower ROS production compared to parental cells. Our novel method successfully induced cancer stem-like cells, which possessed chemoresistance that was related to the cell cycle, drug efflux, and ROS.
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| Free Research Field |
腫瘍学
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