2014 Fiscal Year Final Research Report
The role of unfolded protein response on hepatic stellate cell in old liver ischemia reperfusion injury and liver regeneration
| Project/Area Number |
25861194
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | The University of Tokushima |
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Principal Investigator |
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| Research Collaborator |
SHIMADA Mitsuo 徳島大学, 大学院ヘルスバイオサイエンス研究部, 教授 (10216070)
IMURA Satoru 徳島大学, 病院, 特任教授 (90380021)
MORINE Yuji 徳島大学, 大学院ヘルスバイオサイエンス研究部, 講師 (60398021)
OYADOMARI Seiichi 徳島大学, 疾患プロテオゲノム研究センター, 教授 (90502534)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 肝星細胞 / 加齢肝 / 肝再生 / 小胞体ストレス |
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| Outline of Final Research Achievements |
The method of hepatic stellate cell (HSC) isolation from young and old mouse liver with low-speed centrifugation was established. Gene expression of liver regeneration related genes including HGF and TGFβ. The expression of HGF mRNA in old HSC was significantly lower than that in young HSC. Conditioned medium from old HSC promoted less liver regeneration compared with that from young HSC. For analysis of the difference in unfolded protein response (UPR) of them, HSC was stimulated with stress inducer. The expression of Bip (GRP78) which is sensor of UPR in old HSC was similar with young HSC.
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| Free Research Field |
消化器外科
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