2014 Fiscal Year Final Research Report
Effects of QX-314 on the spinal dorsal horn neurons
| Project/Area Number |
25861363
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Anesthesiology
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| Research Institution | Niigata University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 局所麻酔薬 / 脊髄 / パッチクランプ / QX-314 |
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| Outline of Final Research Achievements |
I examined the effects of QX-314 on rat dorsal horn neurons, where primary afferents terminate. First, I used whole-cell patch clamp recording from the dorsal horn neurons of transverse spinal cord slices. QX-314 decreased the amplitude of N-methyl-D-asparate induced currents and of the exitatory postsynaptic currents evoked by the dorsal root stimulation. QX-314, however, affected neither the frequency nor the amplitude of spontaneous excitatory postsynaptic current. Then, I used in vivo extracellular recording from the rat spinal cord. QX-314 decreased the frequency of action potentials induced by noxious stimulation to the rat hindpaw, especially in nociception specific neurons. These results suggest that QX-314 may act as an analgesic drug by inhibition of excitatory responses in dorsal horn neurons.
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| Free Research Field |
麻酔科学
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