2014 Fiscal Year Final Research Report
Study on relationship of change of expression of meiosis control proteins and aging in human oocytes.
| Project/Area Number |
25861464
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Akita University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 減数分裂 / コヒーシン蛋白 / 体外成熟培養 / ヒト卵子 / 卵子老化 / REC8 |
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| Outline of Final Research Achievements |
We examined the immature oocytes of 17 cases with endometrioid adenocarcinoma. Patients of this study are 35-43 years old and are the age group where pregnancy rate greatly decreases. This data are important in examining human oocytes quality and association of aging. We obtained 166 oocytes in this study. It was found that we could collect human oocytes from resected ovaries effectively. We conducted in vitro maturation for the immature oocytes and, we were able to mature them to meiosis II stage. We made clear that retrievable oocytes from ovaries significantly decreased in age dependency. We reported these in JARG in 2013.
Also, we examine it for the expression level of the cohesin protein (REC8, SGOL2). We evaluated them using fluorescence immunostaining as a method of the fixed-quantity evaluation in Relative Intensity. We reported the above in Sanka-to-Fujinka, 2015 (in Japanese). Also, we conducted oral presentation in International Federation of Fertility Societies of 2015.
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| Free Research Field |
産婦人科学、生殖内分泌学
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