Identification of novel biomarker candidates for immunohistochemical diagnosis of biliary atresia using a proteomic approach

2014 Fiscal Year Final Research Report

• Project/Area Number: 25861666
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pediatric surgery
• Research Institution: Mie University
• Principal Investigator: OTAKE Kohei 三重大学, 医学部, 助教 (40378344)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 胆道閉鎖症 / バイオマーカー / プロテオミクス / shotgun proteomics / MRM / 免疫組織学的染色

Outline of Final Research Achievements

Biliary atresia is a rare pediatric liver disease of unknown etiology and unpredictable outcome, even when there has been timely diagnosis and surgery. Intraoperative cholangiography is the only reliable method to diagnosis biliary atresia definitely. The aim of this study was to identify a novel biomarker for diagnosing biliary atresia. Fifty-eight proteins, including ANXA2，BHMT，CTSD and HMGCS2, were only detected in biliary atresia using shotgun proteomic analysis. Each proteotypic peptide of ANXA2，BHMT，CTSD and HMGCS2 was detected at a significantly higher level in biliary atresia by multiple reaction monitoring. Immunohistochemistry revealed that the expression levels of ANXA2，BHMT，CTSD and HMGCS2 were significantly higher in the liver tissues of patients with biliary atresia. In conclusion, the detected proteins, including ANXA2，BHMT，CTSD and HMGCS2, could be potential biomarkers for diagnosing biliary atresia.

Free Research Field

小児外科