2015 Fiscal Year Final Research Report
study of voltage-applied unfrozen storage of tissue
| Project/Area Number |
25861711
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plastic surgery
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| Research Institution | Kansai Medical University |
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Principal Investigator |
kuro atsuyuki 関西医科大学, 医学部, 助教 (20509492)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 氷点下非凍結保存 / 組織保存 / 再接着 |
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| Outline of Final Research Achievements |
Study of voltage-applied unfrozen storage of tissue. Rat femoral artery was preserved under the conditions of 4 ℃ 0V and -2 ℃ 1000V. It was observed vascular endothelial cells in a transmission electron microscope. Endothelial cells were dissected under the conditions of 4 ℃ 0V in 3 days. The viability of the blood vessel for 3 days preserved rat aorta using a CCK that can measure cell viability was assessed. -2 ℃ 1000V conditions were significantly survival. 3 days preserved rat femoral artery was performed bypass graft to the same strain rats. Good trend at -2 ℃ 1000V conditions came.
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| Free Research Field |
形成外科
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