Photodynamic therapy for methicillin-resistant staphylococcus aureus infected burn wound

2014 Fiscal Year Final Research Report

• Project/Area Number: 25861726
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Emergency medicine
• Research Institution: Osaka City University
• Principal Investigator: MORIMOTO Kuniyuki 大阪市立大学, 医学(系)研究科(研究院), 医員 (10648562)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 光線力学療法 / MRSA / 5-アミノレブリン酸 / 熱傷

Outline of Final Research Achievements

An in vitro study, total porphyrin production in MRSA was increased three times from P. aeruginosa when 5-ALA was added, and 16% were protoporphirin 9 in quantity of porphyrin. It decreased from 16% to 11% in the MRSA whereas PpⅨ rose 7 times in P. aeruginosa when ALA and EDTA were added simultaneously. This result shows administration of 5-ALA alone was effective without adding EDTA in PDT for MRSA. An in vivo study, we demonstrated that PpⅨ successfully accumulated in MRSA on the ulcer surface after intraperitoneal administration of 5-ALA to mice. Furthermore, we found that ALA-PDT accelerated wound healing. Our findings indicate that ALA-PDT may be a new treatment option for MRSA-infected wound.

Free Research Field

形成外科