Leukocyte-rich plasma as a specimen for the measurement of endotoxin

2014 Fiscal Year Final Research Report

• Project/Area Number: 25861730
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Emergency medicine
• Research Institution: Iwate Medical University
• Principal Investigator: KAN SHIGENORI 岩手医科大学, 医学部, 助教 (80633081)
• Co-Investigator(Renkei-kenkyūsha): INADA Katuya 岩手医科大学, 医学部, 助教授 (80048446) ; TAKAHASHI Gaku 岩手医科大学, 医学部, 助教 (60453304) ; TANPO Akihito 岩手医科大学, 医学部, 助教 (80531524) ; KOJIKA Masahiro 岩手医科大学, 医学部, 助教 (40347878)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 感染症 / 敗血症 / エンドトキシン / 多白血球血漿

Outline of Final Research Achievements

We focused our attention on the endotoxin present within and on the surface of white blood cells and attempted to establish a new sample preparation method for endotoxin assays in leukocyte-rich plasma (LRP), taking advantage of the erythrocyte-aggregating property of hydroxyethyl starch (HES). We used an endotoxin-specific turbidimetric kinetic assay, which is the conventional method used to assay endotoxin levels in platelet-rich plasma (PRP). Then, we comparatively assessed the assay results obtained with the endotoxin assay using PRP and LRP. It was found that the sensitivity of endotoxin assay in the diagnosis of infections caused by Gram-negative bacteria. These results suggest that our newly developed leukocyte-rich plasma endotoxin assay may contribute to an improvement in the rate of sepsis diagnosis.

Free Research Field

救急医学