2015 Fiscal Year Final Research Report
Suppression of IL-6 gene and protein expression by PEEP-like stretching of human pulmonary artery endothelial cells
| Project/Area Number |
25861731
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Emergency medicine
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | VILI / 遺伝子発現解析 / 伸展刺激 / PEEP |
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| Outline of Final Research Achievements |
The time courses of interleukin (IL)-6 gene expression and protein production were examined in human pulmonary artery endothelial cells (HPAECs) subjected to stretching comprising excessive elongation and slight elongation (excessive plus PEEP-like stretching). The findings that the peak value of IL-6 gene expression induced by excessive plus PEEP-like stretching was lower than that induced by excessive stretching, and that expression of the protein was suppressed by excessive plus PEEP-like stretching, suggest that PEEP-like stretching reduces IL-6 gene expression and that the small degree of IL-6 gene induction is insufficient for synthesis of IL-6 protein. Thus, PEEP-like cyclic stretching suppresses the expression of both the IL-6 gene and protein.
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| Free Research Field |
細胞情報工学
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