A novel transcriptional regulator, SptA, globally regulates pathogenic factors in Staphylococcus aureus

2014 Fiscal Year Final Research Report

• Project/Area Number: 25861744
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Morphological basic dentistry
• Research Institution: Hiroshima University
• Principal Investigator: KATO FUMINORI 広島大学, 医歯薬保健学研究院(歯), 助教 (70452589)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 遺伝子発現 / 転写調節因子 / 病原性 / 黄色ブドウ球菌

Outline of Final Research Achievements

This research demonstrated that a novel transcriptional factor, SptA:Staphylococcal pathogenicity-related transcriptional factor A, globally regulate the production of pathogenicity factors by repressing both of the agr and rnaiii expression in S. aureus. Analysis of variants with amino acid substitutions in the SIS domain revealed that Thr184 and Glu191 are essential amino acid residues for the function of SptA, and further investigation suggested that SptA works as a repressor upon binding with MurNAc-6-phosphate. Furthermore, GFP(Green Fluorescent) variants with different colors and RCFP(Reef Coral Fluorescent Protein) were improved by amino acid substitutions and a codon optimization to enhance the fluorescence brightness in S. aureus.

Free Research Field

細菌学　分子生物学