Study of genome copy number alterations in oral cancer by FNA-FISH

2016 Fiscal Year Final Research Report

• Project/Area Number: 25861913
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Myo Kunihiro 東京医科歯科大学, 歯学部附属病院, 医員 (50634119)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 遺伝子解析 / 口腔扁平上皮癌 / FNA-FISH / SNPマイクロアレイ / ゲノムコピー数変異 / CNA

Outline of Final Research Achievements

In oral tongue cancer, lymph node metastasis is poor-prognostic factor. To understand the mechanisms underlying metastasis, we compared the genetic profiles of paired primary tumor (PT) and metastatic lymph node (MLN) samples in oral tongue cancer patients.
We extracted genomic DNA from formalin-fixed and paraffin-embedded (FFPE) tissue sections of paired PT and MLN samples. We applied the past experience of molecular genetic analysis by FNA-FISH, large-scale genetic profiling was performed using high-density single-nucleotide polymorphism (SNP) microarray. Then, we compared the genetic profiles of PT and MLN samples to identify specific copy number alterations (CNA). As a result, we identified 20q11.2 gain was significantly more frequent in MLN samples. Moreover, the expression of one of 20q11.2 genes, E2F1, was significantly increased during process of metastasis. This study indicates that additional genetic changes may be required for the metastatic process.

Free Research Field

医歯薬学