Basic study on novel bone regeneration medicine using a periodontal ligament cell sheet cultured on an amniotic membrane

2015 Fiscal Year Final Research Report

• Project/Area Number: 25861966
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Amemiya Takeshi 京都府立医科大学, 医学(系)研究科(研究院), 助教 (90398389)
• Research Collaborator: Honjo Ken-ichi 京都府立医科大学, 医学部附属病院, 専攻医 (00756877) ; KUMAMOTO Sonoko 京都府立医科大学, 医学部附属病院, 専攻医 (90613563) ; ADACHI Tetsuya 京都府立医科大学, 医学部附属病院, 専攻医 (10613573) ; YAMAMOTO Kenta 京都府立医科大学, 医学研究科, 助教 (00636160) ; NAKAMURA Takahiro 京都府立医科大学, 医学研究科, 准教授 (30411078) ; YAMAMOTO Toshiro 京都府立医科大学, 医学研究科, 講師 (40347472) ; KANAMURA Narisato 京都府立医科大学, 医学研究科, 准教授 (70204542)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 移植・再生医学 / 再生医学 / 細胞・組織 / 歯学 / 臨床

Outline of Final Research Achievements

The bone differentiation of periodontal ligament cells (PDL cells) was induced on an amniotic membrane. PDL cells grown on the amniotic membrane formed a single cell sheet, with the expressions of cell proliferation, mesenchymal cell, and osteoblast markers, intercellular adhesion molecules, and basement membrane components detected by immunostaining. The sheet formed a calcified cell substrate when transplanted into a laboratory animal. The sheet cultured on the amniotic membrane had a bone differentiation potential, suggesting its applicability to novel bone regenerative medicine.

Free Research Field

歯科・外科系歯学