2016 Fiscal Year Final Research Report
Local gene induction system in an amphibian and its application to morphogenetic regeneration
| Project/Area Number |
25870058
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
Morphology/Structure
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| Research Institution | Hirosaki University (2014-2016)
Tohoku University (2013) |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 再生 / 両生類 / 遺伝子発現 / 四肢 |
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| Outline of Final Research Achievements |
Amphibians have high regenerative capacity. For example, they can regenerate a limb after amputation. However, difficulty in manipulating gene expression in spatially restricted manner has so far hindered elucidation of the molecular mechanisms of regeneration in amphibians. In this study, I applied two types of gene induction systems, IR-LEGO and temperature stimulator to amphibians and enabled local gene induction. As a result, function of a specific gene has been revealed by single cell level gene induction by IR-LEGO. Furthermore, gene induction exclusively in a specific region in a limb has been done by using temperature stimulator.
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| Free Research Field |
発生生物学、再生生物学
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