2014 Fiscal Year Final Research Report
Molecular pharmacological characterization of GRK2 control mechanism in endothelial dysfunction
| Project/Area Number |
25870258
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General pharmacology
Applied pharmacology
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| Research Institution | Hoshi University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 血管内皮障害 / 糖尿病 / GRK2 |
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| Outline of Final Research Achievements |
Many various complications (cardiovascular disease and atherosclerosis etc.) are caused by affection of long-term diabetes. The vascular disorder is the characteristic in common. This leads to impaired endothelial function, which has become a serious public-health threat, and so we designed the present study to elucidate mechanism to control the vascular function, and to suggest that GRK2 (G protein coupled receptor kinase 2) uncovers new molecular targets for the treatment of diabetic complications. As a result, we found that in the endothelium under high glucose condition, GRK2 was up-regulated by the stimulation of insulin or angiotensin II, and let to a stronger inhibition of insulin-induced Akt/eNOS/NO production pathway. Furthermore, we confirmed that the endothelium-dependent vasorelaxation that attenuated in diabetes was improved by giving GRK2 siRNA to spontaneous type 2 diabetes mice, suggesting that GRK2 is a precipitating factor on the development of diabetic complications.
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| Free Research Field |
薬学
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