Development of phospholamban aptamer as a novel therapeutic tool for treatment of heart failure

2014 Fiscal Year Final Research Report

• Project/Area Number: 25870470
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General pharmacology; Applied pharmacology
• Research Institution: Yamaguchi University
• Principal Investigator: SAKAI Hiroki 山口大学, 医学(系)研究科(研究院), 助教 (40464367)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: ホスホランバン / アプタマー / 心不全治療薬 / 細胞膜透過性ペプチド

Outline of Final Research Achievements

We tried to develop phospholamban aptamer as a novel therapeutic tool for treatment of heart failure. We conjugated the phospholamban RNA aptamer with a cell-penetrating peptide, the modified TAT peptide. This cell-penetrating phospholamban aptamer enhanced contraction/relaxation of isolated adult rat cardiomyocytes as well as corresponding Ca2+ transients. In the mouse heart failure model, the cell-penetrating phospholamban aptamer improved cardiac functions some mice. These results suggested that the cell-panetrating phospholamban aptamer is a potential therapeutic tool for treatment of heart failure.

Free Research Field

薬理学