The association between acute lung injury and microRNA expressions in macrophages

2015 Fiscal Year Final Research Report

• Project/Area Number: 25870620
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Emergency medicine; Anesthesiology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Takeshita Jun 京都府立医科大学, 医学(系)研究科(研究院), 客員講師 (40433263)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: micro RNA / 肺傷害

Outline of Final Research Achievements

We focused on microRNA expressions in macrophages and monocytes in the presence of acute lung injury (ALI) or acute respiratory distress syndrome (ARDS). In the first step, we prepared a macrophage (and THP-1 cell) inflammatory model by lipopolysaccharide (LPS) exposure, and analyzed microRNA expressions. In the second step, we investigated the changes in intracellular signaling molecules (e.g., TNF-α) with the transgenesis of microRNA using Nucleofector. The results of the first step showed the altered expression of miR-21 based on comprehensive analysis and the real-time PCR method. MiR-21 transgenesis induced the increased expressions of IL-10 and P-Akt (P308) proteins based on Western blotting.

Free Research Field

周術期学