Photodynamic diagnosis and therapy of peritoneal dissemination for pancreatic cancer using 5-ALA.

2014 Fiscal Year Final Research Report

• Project/Area Number: 25870622
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery; Life / Health / Medical informatics
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: MURAYAMA Yasutoshi 京都府立医科大学, 医学(系)研究科(研究院), 助教 (50578979)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 膵癌 / 腹膜播種 / 光線力学的診断 / 光線力学的治療 / 5-ALA

Outline of Final Research Achievements

5-aminolevulinic acid (5-ALA) is a natural animo acid and heme precursor. 5-ALA is converted to protoporphyrin IX (PpIX) that is photosesitizer. In this study, we evaluated the usefulness of photodynamic diagnosis (PDD) and photodynamic therapy (PDT) using 5-ALA. Fluorescence laparoscopy was performed in 18 patients with advanced pancreatic cancer. Two of the 18 patients demonstrated by fluorescence laparoscopy. All lesions were diagnosed as peritoneal disseminations by hematoxylin and eosin staining.
The human pancreas cancer cell lines were used both in vitro and in vivo. After 5-ALA administration, cells were irradiated using various LEDs. After irradiation, the cytotoxic effects of ALA-PDT were measured using the MST assay. After 5-ALA administration xenograft mice were irradiated with LEDs. ALA-PDT was repeated three times at weekly intervals. Tumor weights were measured. Relative to controls, ALA-PDT using LEDs showed significant anti-tumor effects both in vitro and in vivo.

Free Research Field

医歯薬学