2015 Fiscal Year Final Research Report
Epidemiological study about water borne protozoa in wild deer in Japan
Project/Area Number |
25871155
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Epidemiology and preventive medicine
Hygiene and public health
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Research Institution | Iwate University (2015) National Institute of Health Sciences (2013-2014) |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Keywords | 水系病原性微生物 / 野生動物 / シカ |
Outline of Final Research Achievements |
In this study we examined about water borne protozoa in wild deer in Japan. 251 fecal samples were collected from 9 prefectures and 5 water samples from 5 different areas in 1 prefecture. As a result, we detected Cryptosporidium positive fecal samples from 5 prefectures, those positive rates were from 2% to 20% for each. Positive rates of male was 8.9%, and that of female was 4.1%. 2 water samples were Cryptosporidium positive among 5 samples. Nucleotide sequencing analysis of 18S ribosomal RNA detected C. ryanae, C. bovis, and C. sp. deer genotype from fecal samples, in other hand, C. fragile and C. galli were detected from water samples. In discussion, we detected Cryptosporidum sp. in wild deer and water samples which were collected in same area but species were genetically different so that wild deer is not a direct source of water pollution. But those species from deer is predominant for cattle that indicates deer might be a vector of infectious Cryptosporidium spp. for cattle.
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Free Research Field |
公衆衛生
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