2015 Fiscal Year Final Research Report
Differential proteome analysis identifies TGF-beta related pro-metastatic proteins in a 4T1 murine breast cancer model
| Project/Area Number |
25871241
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Tumor biology
Genome biology
|
|---|
| Research Institution | Osaka City University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Keywords | TGF-beta / Proteomics / eIF signaling / metastatic mouse model / Breast cancer |
|---|
| Outline of Final Research Achievements |
Although TGF-β antagonists have been proposed as anti-metastatic therapies for patients with advanced stage cancer, how TGF-β mediates metastasis-promoting effects is poorly understood. In the present study, we found that systemic administration of the TGF-β receptor kinase inhibitor, SB-431542, significantly inhibited lung metastasis from transplanted 4T1 mammary tumors in Balb/c mice. The differentially expressed proteins in the comparison of lung metastases from SB-431542 treated and control vehicle-treated groups were analyzed by proteomics analysis. Our proteomic approach newly identified eIF pathway proteins as novel potential mediators of TGF-β tumor-promoting activity.
|
| Free Research Field |
分子生物学
|
