In vivo measurement of mitochondrial function in human skeletal muscle

2014 Fiscal Year Final Research Report

• Project/Area Number: 25882041
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Sports science
• Research Institution: Ritsumeikan University
• Principal Investigator: SUGA Tadashi 立命館大学, 立命館グローバル・イノベーション研究機構, 研究員 (30708673)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Keywords: 運動 / 骨格筋 / ミトコンドリア機能 / 磁気共鳴分光法 / 近赤外分光法

Outline of Final Research Achievements

The present study aimed to examine the availability of a noninvasive method for evaluating mitochondrial function in skeletal muscle by combining phosphorus magnetic resonance spectroscopy (31P-MRS) and near-infrared spectroscopy (NIRS). Ischemia of the lower limb was induced for 15 min by using a rapid inflator with a pressure cuff placed around the thigh. Under this condition, the reduced speeds of phosphocreatine (PCr) and oxy-hemoglobin/myoglobin (Hb/Mb) were measured using 31P-MRS and NIRS, respectively. The rate of these speeds was calculated as mitochondrial coupling capacity. The present study found that the reduced speeds of PCr and oxy-Hb/Mb were significantly higher in endurance-trained subjects than in untrained subjects, whereas mitochondrial coupling capacity did not differ significantly between endurance-trained subjects and untrained subjects. Thus, mitochondrial coupling capacity may not be appropriate in evaluating mitochondrial function in skeletal muscle.

Free Research Field

健康科学