Development of CRISPR/Cas system enabling advanced genome editing on chromosome level

2014 Fiscal Year Final Research Report

• Project/Area Number: 25890014
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: System genome science
• Research Institution: Hiroshima University
• Principal Investigator: SAKUMA TETSUSHI 広島大学, 理学(系)研究科(研究院), 特任助教 (90711143)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Keywords: ゲノム編集 / CRISPR/Cas9

Outline of Final Research Achievements

We developed a vector system enabling advanced genome editing such as simultaneous disruption of multiple genes and large chromosomal deletion, and evaluated its applicability in cultured cells and mice. In cultured cells, multiple targeted mutagenesis for up to seven genes and chromosomal deletion were demonstrated. In addition, it was shown that simultaneous gene disruption at multiple gene loci was feasible in mouse embryos by single plasmid injection using an all-in-one vector carrying FokI-dCas9, which is known as a highly-specific variant of Cas9.

Free Research Field

ゲノム生物工学