Regulation of celullar differentiation by degradation of core promoter recognition complex

2014 Fiscal Year Final Research Report

• Project/Area Number: 25891003
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: Tohoku University
• Principal Investigator: NAKAGAWA TADASHI 東北大学, 医学(系)研究科(研究院), 助教 (30707013)
• Research Collaborator: FUNAYAMA Ryo 東北大学, 大学院医学系研究科, 助教 (20452295) ; HOSOGANE Masaki 東北大学, 大学院医学系研究科, 助手 (30734347)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Keywords: 発生・分化 / 上皮間葉移行 / タンパク質分解 / シグナル伝達

Outline of Final Research Achievements

Cell differentiation accompanies global gene expression change. Previous studies revealed that genes to be expressed are determined by transcription factors, but emerging evidences indicate that the general transcription machinery is also involved in gene expression change.
The general transcription machinery comprises several complexes, among which the promoter recognition complex TFIID plays a pivotal role in the selection of expressed genes. During the differentiation of myoblastic cell line and the epithelial-to-mesenchymal transition (EMT) of mammary epithelial cell line, several components of TFIID were found to be degraded by the ubiquitin-proteasome system. Induction of EMT caused the increase in an ubiquitin ligase which promoted the degradation of the TFIID component, leading to the reduction of translation-related gene expression. These results revealed a novel mechanism of regulation of the general transcription machinery by protein degradation during cell differentiation.

Free Research Field

分子生物学