2014 Fiscal Year Final Research Report
The evaluation of the promotion of secondary dentin by controlling release of simvastatin
| Project/Area Number |
25893264
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
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| Project Period (FY) |
2013-08-30 – 2015-03-31
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| Keywords | 徐放 / シンバスタチン / 歯髄細胞 / 象牙芽細胞 / 硬組織再生 |
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| Outline of Final Research Achievements |
Simvastatin (ST) was incorporated into the micelles of gelatin grafted with L-lactic acid oligomers (LAo) to allow water-solubilization. The simvastatin-LAo-grafted gelatin (LAo-g-gelatin) micelles were mixed with gelatin, followed by chemical crosslinking to form gelatin hydrogels (ST Mi/GH). The ST Mi were released from the gelatin hydrogel granules (GH) through enzymatic degradation. From the in vitro experiments, the most effective ST Mi/GH for odontoblastic differentiation was that of 2 weeks degradation. In addition, the ST in the micelle form exhibited biological activities to induce ALP activity, calcium deposition and BMP-2 production in vitro. Now the in vivo experiments are evaluated. The quality and quantity of dentin tissue formed should be evaluatedusing dental pulp capping models and comparing with those of other pulp-capping materials.
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| Free Research Field |
drug delivery system
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