Role of NBS1 C-terminal domain in genomic stability

2017 Fiscal Year Final Research Report

• Project/Area Number: 26241013
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Kyoto University
• Principal Investigator: Komatsu Kenshi 京都大学, 放射線生物研究センター, 研究員 (80124577)
• Co-Investigator(Kenkyū-buntansha): 小林 純也 京都大学, 放射線生物研究センター, 准教授 (30301302) ; 田内 広 茨城大学, 理学部, 教授 (70216597) ; 柳原 晃弘 東北医科薬科大学, 医学部, 助教 (70423051) ; 松浦 伸也 広島大学, 原爆放射線医科学研究所, 教授 (90274133)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: NBS1 / クロマチン・リモデリング / 損傷乗越えDNA合成 / 非相同末端再結合 / ドメイン解析

Outline of Final Research Achievements

We had reported several functional domains at the C-terminla of NBS1, which was identified as a protein responsible for radiation sensitive disease, Nijmegen breakage syndrome, by us in 1998. The functional roles of these domains were analyzed by using the cells lacking the specified domain or knock-in mice, which revealed the association with the radiation resistance at low dose irradiation in radiation therapy and with the genome instability, such as mutagenesis and carcinogenesis. Moreover, we found two novel functional domains in the C-terminal region and middle region, respectively. Analysis using CRISPRA technique indicated their functional roles in non-homologous end-joining for radiation-induced DNA double strand breaks and also in another genome stability.

Free Research Field

放射線生物学