Chromatin dynamics induced by inflammatory stimulation

2016 Fiscal Year Final Research Report

• Project/Area Number: 26250041
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: System genome science
• Research Institution: The University of Tokyo
• Principal Investigator: Wada Youichiro 東京大学, アイソトープ総合センター, 教授 (10322033)
• Co-Investigator(Kenkyū-buntansha): 神吉 康晴 東京大学, アイソトープ総合センター, 助教 (00534869) ; 藤井 穂高 大阪大学, 微生物病研究所, 准教授 (30302665) ; 興梠 貴英 自治医科大学, 医学部, 准教授 (40401046)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 転写 / RNAポリメレースII / 炎症 / 内皮細胞

Outline of Final Research Achievements

Among vascular diseases, atherosclerosis is one of the most important factors of ischemic heart diseases and cerebrovascular diseases, and elucidation of the mechanism in consistent gene expression regulation of atherogenic proteins. To dissect the adhesion molecule gene regulation, we adopted a model using endothelial cells stimulated inflammatory stimulator, and presence of transcription complexes, which regulate multiple genes at the same time, was suggested. In this research, we performed (i) comprehensive chromatin interaction analysis, (ii) identification of interaction associated proteincomplex, (iii) intervention of chromatin structure, and it was observed that transcription factories regulated both induced and reduced genes from different chromosomes.

Free Research Field

内科循環病学