Molecular basis for meiotic recombination of homologous chromosomes

2016 Fiscal Year Final Research Report

• Project/Area Number: 26251037
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Chromosome dynamics
• Research Institution: Osaka University
• Principal Investigator: HIRAOKA Yasushi 大阪大学, 生命機能研究科, 教授 (10359078)
• Co-Investigator(Renkei-kenkyūsha): ASAKAWA Haruhiko 大阪大学, 生命機能研究科, 准教授 (70399533)
• Research Collaborator: CHIKASHIGE Yuji 国立研究開発法人情報通信研究機構, 未来ICT研究所, 主任研究員 (60359081) ; OBUSE Chikashi 北海道大学, 先端生命科学研究院, 教授 (00273855)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 細胞

Outline of Final Research Achievements

Imaging of meiotic chromatin: Using super-resolution microscopy, we found that meiotic cohesion-based chromatin structures are necessary for homologous chromosome pairing. We also found that chromatin decompaction occurs during meiotic DNA replication and that the decompaction requires acetylation of histone H4. In addition, we developed a new method to visualize histone modification in living cells.
Regulatory mechanisms of meiosis: We found that a component of the nuclear pore complex, Nup132, plays an important role in progression of meiosis, and that two APC/C activators regulate progression of meiosis. We also revealed that a nuclear membrane protein Lem2 augments centromeric heterochromatin in a nutrition-dependent manner.

Free Research Field

分子細胞生物学